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Human DPPII / QPP / DPP7 Protein  pdf  pdf  pdf


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DP7-H5222-50ug
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Synonym

DPPII,QPP,DPP7,DPP2,Dipeptidyl peptidase 2,Dipeptidyl aminopeptidase II

Source

Human DPPII, His Tag (DP7-H5222) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Leu 492 (Accession # AAH11907).

Predicted N-terminus: Gly 22

Molecular Characterization

DPPII(Gly 22 - Leu 492)AAH11907
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 53 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human DPPII, His Tag (Catalog # DP7-H5222) SDS-PAGE gel

Human DPPII, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Dipeptidyl peptidase 2 (DPP2 or DPP II) is also known as Dipeptidyl peptidase 7 (DPP7), Quiescent cell proline dipeptidase (QPP), which belongs to the peptidase S28 family. DPP7 / DPP2 is detected in seminal plasma (at protein level). When the pH is 5.5, Dipeptidyl peptidase 2 display a optimum catalytic activity. DPP7 has low activity at pH 7 and is inactive at pH 8. DPP7 plays an important role in the degradation of some oligopeptides.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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