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Human OMgp / OMG Protein  pdf  pdf  pdf


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Synonym

OMG, OMGP

Source

Recombinant Human OMG /OMGP Protein (rh OMG / OMGP) Ile 25 - Asn 420 (Accession # AAH18050) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization

rh OMG / OMGP, fused with polyhistidine tag at the C-terminus, has a calculated MW of 45.5 kDa. The predicted N-terminus is Ile 25. DTT-reduced Protein migrates as 90-120 kDa due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh OMG / OMGP by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human OMG /OMGP Protein
The purity of rh OMG / OMGP was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Oligodendrocyte-myelin glycoprotein (OMG or OMGP), is a cell membrane protein which contains eight LRR (leucine-rich) repeats and one LRRNT domain. OMG / OMGP is a glycosylphosphatidylinositol-anchored protein expressed by neurons and oligodendrocytes in the central nervous system (CNS). OMG / OMGP play roles in both the developing and adult central nervous system. OMG / OMGP may also be involved in the formation and maintenance of myelin sheaths.

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References

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