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Your Position: Home > Protein > PD-L2 > PD2-H5251

Human PD-L2 / B7-DC Protein, Fc Tag (HPLC verified)

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  • Synonym
    PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2
  • Source
    Human PD-L2 Protein, Fc Tag(PD2-H5251) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).
    Predicted N-terminus: Leu 20
  • Molecular Characterization
    PD-L2 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
PD-L2 SDS-PAGE

Human PD-L2 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-HPLC
PD-L2 SEC-HPLC

The purity of Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) was greater than 90% as determined by SEC-HPLC.

Bioactivity-BLI
 PD-L2 BLI

Loaded Human PD-1, His Tag (Cat. No. PD1-H5221) on HIS1K Biosensor, can bind Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

 PD-L2 BLI

Loaded Human PD-1 Protein, His Tag (Cat. No. PD1-H522a) on HIS1K Biosensor, can bind Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 PD-L2 BLI

Loaded Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) on Protein A Biosensor, can bind Human PD-1 Protein, His Tag (Cat. No. PD1-H522a) with an affinity constant of 0.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 PD-L2 BLI

Loaded Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) on Protein A Biosensor, can bind Human PD-1 Protein, Twin-Strep Tag (Cat. No. PD1-H5284) with an affinity constant of 0.42 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-FACS
 PD-L2 FACS

Flow Cytometry assay shows that recombinant Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 μg/mL (Routinely tested).

Bioactivity-Bioactivity CELL BASE
 PD-L2 CELL

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) with an EC50 of 0.018 μg/mL (Routinely tested).

  • Background
    Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
  • Clinical and Translational Updates

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