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Human PD-1 / PDCD1 Protein, Tag Free, Full Length  pdf  pdf  pdf


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PD1-HC214-20ug
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Synonym

PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l

Source

Recombinant Human full length PDCD1 Protein, Tag Free (rh PDCD1 /PD1 (full length)) Leu 25 - Leu 288 (Accession # NP_005009.2) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

PD-1 (Leu 25-Leu 288)NP_005009.2

rh PDCD1 /PD1 (full length) contains no "tag", and has a calculated MW of 29.3 kDa. The predicted N-terminus is Leu 25. DTT-reduced Protein migrates as 29-70 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh PDCD1 /PD1 (full length) by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5 with detergent. Normally Trehalose is added as protectant before lyophilization.

Contact us for customized product format or formulation.

Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE

Human PD-1, Tag Free, Full Length on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity

Immobilized Human PD-1, Tag Free, Full Length (Catalog # PD1-HC214) at 0.2 μg/mL ( 100 μl/well ) can bind Biotinylated Human PD-L1, His Tag & Fc Tag (Catalog # PD1-H82F3) with a linear range of 0.08-2.5 μg/mL.

Western Blot

 

  • Lane1, Negtive control, 10 μg HEK 293 cell lysate
  • Lane2, 10 ng rhPDCD1 full length protein in 10 μg HEK293 lysate
  • Lane3, 100 ng rhPDCD1 full length protein in 10 μg HEK293 lysate
  • Lane4, Positive control, 100 ng rhPDCD1 protein (Catalog # PD1-H5221) in 10 μg HEK293 lysate

Detection by anti-human PDCD1 mAb (4H1)

 

Bioactivity

Immobilized Human PD-1, Tag Free, Full Length (Catalog # PD1-HC214) at 0.2 μg/mL ( 100 μl/well ) can bind Biotinylated Human PD-L2, Fc Tag (Catalog # PD2-H82F6) with a linear range of 5-156 ng/mL.

 
 

Background

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


References