Heavy-labeled Products (MS Standards) and Services
|Comparison of ACROBiosystems protein based MS Standard Vs. Other Company peptide based MS standard|
|ACRO protein||Other Company peptide|
|Identification of best SRM and MRM transitions||Yes||No|
|PT modification and processing||Yes||No|
Recombinant Human ADAM12 MS Standard Protein, C13 and N15-labeled (rhADAM12, Heavy Labeled) Arg 29 - Asp 513 (Accession # AAH60804.1) was produced through human 293 cells (HEK293) in chemically defined medium.
Recombinant Human Apo-A1 MS Standard Protein, C13 and N15-labeled (rh Apo-A1, Heavy Labeled) Asp 25 - Gln 267 (Accession # NP_000030.1) was produced through human 293 cells (HEK293) in chemically defined medium.
Heavy-labeled Protein Products
Stable isotope labeling by amino acids in cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. It is a popular method for (MS)-based quantitative proteomics. SILAC labels cellular proteomes through normal metabolic processes, incorporating non-radioactive, stable isotope containing amino acids into the proteins. The method relies on the incorporation of amino acids with substituted stable isotopic nuclei (e.g. 13C, 15N). For example, the growth medium can contain arginine labeled with six carbon-13 atoms (13C), heavy amino acid, instead of the normal carbon-12 (12C), light amino acids. Cells grown in this medium incorporate the heavy amino acids after five cell doublings and SILAC amino acids have no effect on cell morphology or growth rates, they incorporate the heavy amino acid,13C6-arginine, into all of cell proteins(Figure 1).
SILAC has emerged as a very powerful method to study cell signaling, post translation modifications such as phosphorylation, protein–protein interaction and regulation of gene expression. In addition, SILAC has become an important method in secretomics, the global study of secreted proteins and secretory pathways. It can be used to distinguish between proteins secreted by cells in culture and serum contaminants. Standardized protocols of SILAC for various application have also been published.
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