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TIGIT Signaling Axis

TIGIT (T cell immununo receptor with lg and ITIM domains) is a newly discovered immune checkpoint protein expressed on T cells and Natural Killer cells (NK). TIGIT is an inhibitory receptor which modulates the immune activity by competing with activating receptor CD226 (also known as DNAM-1) for ligand binding. This on and off switch of TIGIT & DNAM-1 parallels that of CTLA4 & CD28, which makes it a promising target for anti-tumor therapy.

TIGIT proteins

CD155 (PVR) and CD112 (PVRL2 or Nectin-2) are co-ligands of TIGIT and DNAM-1, while CD113 (PVRL3 or Nectin-3) is only binding to TIGIT. The affinity between different ligands and receptors vary. The binding between TIGIT and CD155 is more robust than that between TIGIT and CD112 or CD113.

ACROBiosystems carries a large collection of recombinant proteins from TIGIT signaling axis. In addition to the commonly used his and Fc tagged proteins, we also carry multiple pre-biotinylated proteins such as Biotinylated Human TIGIT, Avi Tag (Avitag™) (Cat. No. TIT-H82E5), Biotinylated Human CD155 / PVR Protein, Fc Tag, Avi Tag (Avitag™) (Cat. No. CD5-H82F6) and Biotinylated Human Nectin-2 / CD112 Protein, Fc Tag, Avi Tag (Avitag™) (Cat. No. CD2-H82F8). Please find detailed information below:

Bioactivity Data

Fig.1 FACS assay shows Biotinylated Human TIGIT, Fc,Avitag (Cat. No. TIT-H82F1) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT is 0.03 μg/mL.

Fig.2 FACS assay shows that Human TIGIT, Mouse IgG2a Fc tag, low endotoxin (Cat. No. TIT-H5253) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/mL.

Fig.3 Anti-Human TIGIT MAb (Mouse IgG1) captured on CM5 chip via Anti-Mouse antibodies surface, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 3.93 nM as determined in a SPR assay (Biacore T200).

Fig.4 Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, His Tag (Cat. No. TIT-H52H3): Biotinylated Human CD155, Fc Tag (Cat. No. CD5-H82F6) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

TIGIT Product List

Cat. No.SpeciesProduct DescriptionStructurePurityFeatures
TIT-H82E5
(Biotin-labeled)

Biotinylated Human TIGIT Protein, Avi TagTIT-H82E5

TIT-H82F1
(Biotin-labeled)

Biotinylated Human TIGIT Protein, Fc Tag, Avi TagTIT-H82F1

TIT-C5223
Cynomolgus / Rhesus macaque TIGIT ProteinTIT-C5223-STC1

TIT-C5254
Cynomolgus / Rhesus macaque TIGIT Protein, Fc TagTIT-C5254-STC1

TIT-M52E6
Mouse TIGIT Protein, His TagTIT-M52E6-STC1

TIT-M5257
Mouse TIGIT Protein, Fc TagTIT-M5257-STC1

TIT-R5258
Rat TIGIT Protein, Fc TagTIT-R5258-STC1

TIT-R5259
Rabbit TIGIT Protein, Fc TagTIT-R5259-STC1

TIT-H52H3
Human TIGIT Protein, His TagTIT-H52H3-STC1

TIT-H5253
Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxinTIT-H5253-STC1

TIT-H5254
Human TIGIT Protein, Fc TagTIT-H5254-STC1

TIT-C52H7
Canine TIGIT Protein, His TagTIT-C52H7-STC1

Other Relevant Products

MoleculeCat. No.SpeciesProduct DescriptionStructurePurityFeatures
CD155CD5-H82F6
Biotinylated Human CD155 / PVR Protein, Fc, Avi TagCD5-H82F6-STC1

CD5-R52H5
Rhesus macaque CD155 / PVR Protein, His TagCD5-R52H5-STC1

CD5-H5254
Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxinCD5-H5254-STC1

CD5-M5254
Mouse CD155 / PVR Protein, Mouse IgG2a Fc TagCD5-M5254-STC1

CD5-H82E3
Biotinylated Human CD155 / PVR Protein, His, Avitag™CD5-H82E3-STC1

CD5-M82F7
Biotinylated Mouse CD155 / PVR Protein, Fc, Avitag™CD5-M82F7-STC1

CD5-H5251
Human CD155 / PVR Protein, Fc TagCD5-H5251-STC1

CD5-H5223
Human CD155 / PVR Protein, His TagCD5-H5223-STC1
 
CD112CD2-H82F8
Biotinylated Human Nectin-2 / CD112 Protein, Fc, Avi TagCD2-H82F8-STC1

CD2-H5257
Human Nectin-2 / CD112 Protein, Mouse IgG2a Fc TagCD2-H5257-STC1

PV2-H5253
Human Nectin-2 / CD112 Protein, Fc TagPV2-H5253-STC1

PV2-H52E2
Human Nectin-2 / CD112 Protein, His TagPV2-H52E2-STC1

CD113PV3-H82F3
Biotinylated Human Nectin-3 / CD113 Protein, Fc, Avi TagPV3-H82F3-STC1

PV3-H5255
Human Nectin-3 / CD113 Protein, Fc TagPV3-H5255-STC1

PV3-H52E4
Human Nectin-3 / CD113 Protein, His TagPV3-H52E4-STC1

DNAM-1DN1-H82F9
Biotinylated Human DNAM-1 / CD226 Protein, Fc, Avi TagDN1-H82F9-STC1

DN1-C52H9
Cynomolgus DNAM-1 / CD226 Protein, His TagDN1-C52H9-STC1

DN1-H5257
Human DNAM-1 / CD226 Protein, Fc TagDN1-H5257-STC1

DN1-H52H6
Human DNAM-1 / CD226 Protein, His TagDN1-H52H6-STC1

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