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Your Position: Home > B-Cell Maturation Antigen (BCMA), a Target for Multiple Myeloma

B-Cell Maturation Antigen (BCMA), a Target for Multiple Myeloma

BCMA is selectively induced during plasma cell differentiation and is nearly absent on naive and memory B cells[1,2]. The interaction between BCMA and its ligand BAFF and APRIL is involved for the maintenance of long-lived plasma cells [3,4]. BCMA is an ideal target for cancers of plasma cells, because it’s not expressed in other major organs. Dozens of BCMA-targeting therapies, including chimeric antigen receptor T (CAR-T) cells, antibody-drug conjugate, and Bi-specific antibodies, are currently being developed and tested clinically. Leading the pack is Bluebird and Celgene’s bb2121. A phase I multi-center clinical trial has demonstrated a stunning 80% response rate in multiple myeloma patients. These approaches, if successfully established, will bring multiple myeloma patients a much needed option beyond Daratumumab (anti-CD38 antibody) and Elotuzumab (anti-SLAMF7).

BCMA proteins

The interactions between BAFF / APRIL and their receptors are selective. Note BCMA can interact with both BAFF and APRIL[5]. The affinity of APRIL-BCMA interaction, however, is much higher than that of BAFF-BCMA binding[6].

ACROBiosystems provides a comprehensive panel of BCMA proteins, including the exclusive biotinylated BCMA (Cat. No. BC7-H82F0) and the monomeric BCMA (Cat. No. BCA-H522y). We also offer BCMA related proteins, such as BAFF and BAFFR proteins.

Product Validation Data

FACS

293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-labeled human BCMA / TNFRSF17 protein, Fc tag (Cat. No. BCA-HF254, 3 μg/ml) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of FITC-labeled human BCMA / TNFRSF17 protein, Fc tag (Cat. No. BCA-HF254).

Human T cells were transfected with anti-BCMA CAR and cultured for 3 days. Three days post-transfection, 1e6 cells were first incubated with 50ul Biotinylated Human BCMA, Fc,Avitag (Cat.No. BC7-H82F0, 8 μg/ml ), washed and then stained with PE Streptavidin and analyzed by flow cytometry. (Data are kindly provided by PREGENE Biopharma).

BLI
Loaded Anti-Human BCMA MAb (human IgG1) on Protein A Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 35.8 nM as determined in BLI assay (ForteBio Octet Red96e).

SPR
Anti-BCMA MAb (Mouse IgG1, the antibody was co developed by SCT and ACRObiosystems) captured on CM5 chip via anti-mouse antibodies surface can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 0.388 nM as determined in a SPR assay (Biacore T200).
ELISA
Immobilized Human BAFF, Fc Tag (Cat. No. BAF-H4268) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4) with a linear range of 15-125 ng/mL.
Immobilized Human APRIL, Fc Tag (Cat. No. APL-H5267) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4) with a linear range of 0.6-20 ng/mL.

We offer a variety of ready-to-use biotinylated proteins using our in-house developed labeling techniques, which confer high bioactivity and minimal batch-to-batch variation. In addition, we also offer FITC-labeled BCMA and BAFF proteins.

BCMA Product List

MoleculeCat. No.SpeciesStructurePurityFeatures
BCMA
(Biotin-labeled)
BC7-H82F0
BC7-H82F0-STC1

BCMA
(FITC-Labeled)
BCA-HF254
BCA-HF254-STC1

BCMABCA-H522y
BCA-H522y-STC1

BCMABC7-H5254
BC7-H5254-STC1

BCMA
(Biotin-labeled)
BCA-M82F0
BCA-M82F0-STC1

BCMA
(Biotin-labeled)
BCA-M5258
BCA-M5258-STC1

BCMA
(Biotin-labeled)
BCA-C82F4
BCA-C82F4-STC1

BCMABCA-C5253
BCA-C5253-STC1

Other Relevant Products

MoleculeCat. No.SpeciesStructurePurityFeatures
BAFF
(Biotin-labeled)
BAF-H82F3
BAF-H82F3-STC1

BAFF
(Biotin-labeled)
BAF-H82Q2
BAF-H82Q2-STC1

BAFF
(FITC-Labeled)
BAF-HF268
BAF-HF268-STC1

BAFFBAF-H4218
BAF-H4218-STC1

BAFFBAF-H4268
BAF-H4268-STC1

BAFFBAF-H5248
BAF-H5248-STC1

BAFFBAF-M5257
BAF-M5257-STC1

BAFFR
(Biotin-labeled)
BAR-M82F0
BAR-M82F0-STC1

BAFFRBAR-M5259
BAR-M5259-STC1

BAFFRBAR-H5257
BAR-H5257-STC1

APRILAPL-H5267
APL-H5267-STC1

APRILAPL-H5244
APL-H5244-STC1

APRIL
(Biotin-labeled)
APL-H82F5
APL-H82F5-STC1

Other Multiple Myeloma Targets

MoleculeCat. No.SpeciesStructurePurityFeatures
CD38
(Biotin-labeled)
CD8-H82E7
CD8-H82E7-STC1

CD38CD8-H5224
CD8-H5224-STC1

SLAMF7
(Biotin-labeled)
SL7-H82E0
SL7-H82E0-STC1
SLAMF7SL7-H5225
SL7-H5225-STC1
SLAMF7SL7-H5256
SL7-H5256-STC1

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