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Your Position: Home > Specially Designed Proteins for COVID-19 Serological Test Development

Specially Designed Proteins for COVID-19 Serological Test Development

Specially Designed Proteins for COVID-19 Serological Test Development
Background
Accurate diagnosis of COVID-19 is essential

The pandemic caused by SARS-CoV-2 presents a serious global public health emergency. The number of COVID-19 cases and deaths predicted to be rising in the coming weeks and months in the majority of countries. Accurate diagnosis of COVID-19 is not only essential to carry out appropriate patient care but also take timely quarantine measures to limit virus spread.

The demand of serological tests is increasing for COVID-19 diagnose

The widely employed etiological diagnosis of SARS-CoV-2 infection relies on a nucleic acid test with Real Time-PCR (RT-PCR) using specimens collected via nasopharyngeal swab. However, the results show that the positive detection rate of RT-PCR in clinical and epidemiological suspected COVID-19 cases is about 50% due to some factors such as sample quality, virus infection site and sampling time, etc.

Serological tests can be used to identify whether people have been exposed to a particular pathogen by looking for the presence of antibodies that are produced in response to infections. In compared with RT-PCR, serological test usually has the advantages of convenient and stable sample collection, easy realization of high throughput and small workload.

Therefore, the demand for serological tests of SARS-CoV-2 is increasing rapidly in order to improve the accuracy of clinical diagnosis and better quantify the number of cases of COVID-19, including those that may be asymptomatic or have recovered.

The antigens of SARS-CoV-2 are employed in serological test kits

As of May 17, 12 serological test kits for SARS-CoV-2 have received Emergency Use Authorization from FDA, including colloidal gold immunoassay, chemiluminescence immunoassay and ELISA.

The antigens of SARS-CoV-2 are employed in these commercial kits to detect the antibodies present in serum of virus-infected patients. To improve the sensitivity and specificity of serological test kits, antigens derived from the SARS-CoV-2 Spike protein and Nucleocapsid protein are recombinantly produced and used in the antibody test cassette.

ACROBiosystems has developed SARS-CoV-2 antigens specifically designed and optimized for serological test kits:

  1. Customized formulation available

  2. Capable of supplying in bulk order

  3. Functionality confirmed

  4. Multiple tags and biotin label version ready to choose

  5. 5+ RBD mutant proteins

Data

The sensitivity of N protein binding to Anti-N mAb is 0.02 ng/mL as verified by ELISA

Fig.1 Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C51H9) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.3 ng/mL.

The sensitivity of S1 protein binding to ACE2 protein is 0.2 ng/mL as verified by ELISA

Fig.2 Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H4) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL.

The sensitivity of RBD protein binding to ACE2 protein is 8 ng/mL as verified by ELISA

Fig.3 Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with a linear range of 8-125 ng/mL.

Product List
>>> If you have any customized inquiries or suggestions for SARS-CoV-2 (COVID-19) related product development, please click here.
>>> If you have any bulk order inquiry, please feel free to here.
MoleculeCat. No.Product Description
S1 proteinS1N-C52H3SARS-CoV-2 (COVID-19) S1 protein, His Tag
S1N-C82E8Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified)
S1N-S52H5SARS S1 protein, His Tag (MALS verified)
S1N-C52H4SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified)
S1N-C5255SARS-CoV-2 (COVID-19) S1 protein, Fc Tag
S1N-C5257SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag
S protein RBDSPD-C82E9Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)
SPD-C5255SARS-CoV-2 (COVID-19) S protein RBD, Fc Tag (MALS verified)
SPD-S52H6SARS S protein RBD, His Tag (MALS verified)
SPD-C52H3SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)
SPD-C5259SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag
SPD-S52H4SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag
SPD-S52H5SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag
SPD-S52H7SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag
SPD-S52H8SARS-CoV-2 (COVID-19) S protein RBD (R408I), His Tag
SPD-S52H3SARS-CoV-2 (COVID-19) S protein RBD (N354D, D364Y), His Tag
Nucleocapsid proteinNUN-C51H9SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag
NUN-C5227SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag
NUN-C81Q6Biotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™

>>> Check all SARS-CoV-2 proteins and inhibitory kits.

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