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Your Position: Home > COVID-19 R&D > Bead-Based Phagocytosis Assay: Cost-effective, Highly Efficient and Convenient to Use
Bead-Based Phagocytosis Assay: Cost-effective, Highly Efficient and Convenient to Use
Release time: 2021-04-30 Source: ACROBiosystems Read: 870

With the simple aid of a magnetic field, magnetic beads pre-coupled with biotinylated proteins are well-suited for highly efficient immunocapture, biopanning and virus capture. Apart from these common applications, beads can also be used in antibody-dependent cellular phagocytosis (ADCP) assay to evaluate the phagocytic potential of activated effector cells, an experiment commonly performed in tumor or virus studies.

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   Fig.1. Antibody-mediated cellular phagocytosis (ADCP) of protein-coupled beads.

A recent study of HIV-1 reported the use of fluorescent bead-based flow cytometric assay to quantify cellular phagocytosis of GP41-coupled beads by monocytes. In the standard bead ADCP assay they performed, protein-coupled fluorescent beads are incubated with antibodies to be adequately opsonized prior to addition to phagocytic cells, and the beads uptak by cells are analyzed by flow cytometry. To further differentiate the surface-attached beads from the intracellular beads, they incorporated a Specific Hybridization Internalization Probe (SHIP) which selectively inactivate the SHIP fluorescence of the attached beads but not of those truly internalized. In this way, the number of beads phagocytosed by single effector cell can also be accurately identified from the fluorescence level. In summary, they reported the method being highly efficient and repeatable.


In bead-based ADCP assay,detection of the phagocytosed beads can be performed with fluorescence technology or electronic microscopy (EM). ACROBiosystems has developed a list of beads products that are suitable to be used in ADCP assays and conveniently visualized with EM. These 2um beads can provide reliable simulation data by mimicking antigen presentation on different tumor cells or virus-infected cells. Additionally, using bead-based assay to measure Fc-mediated phagocytosis has advantages over real cell assays for being inexpensive, convenient to manipulate and can be stored for a long period of time.


Pre-Coupling Magnetic Beads

Product Lists

MoleculeCat. No.Product DescriptionOrder/Preorder
S proteinMBS-K029SARS-CoV-2 Spike Trimer (B.1.1.7) Coupled Magnetic Beads

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S proteinMBS-K030SARS-CoV-2 Spike Trimer (B.1.351) Coupled Magnetic Beads

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S proteinMBS-K031SARS-CoV-2 Spike Trimer (P.1) Coupled Magnetic Beads

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Spike proteinMBS-K015SARS-CoV-2 Spike Trimer-coupled Magnetic Beads

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Spike proteinMBS-K016SARS-CoV-2 Spike Trimer (D614G)-coupled Magnetic Beads

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S protein RBDMBS-K032SARS-CoV-2 Spike RBD (B.1.351) Coupled Magnetic Beads

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S protein RBDMBS-K033SARS-CoV-2 Spike RBD (P.1) Coupled Magnetic Beads

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S protein RBDMBS-K034SARS-CoV-2 Spike RBD (B.1.1.7) Coupled Magnetic Beads

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Spike RBDMBS-K014Human Anti-SARS-CoV-2 Spike RBD Antibody-coupled Magnetic Beads

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Spike NTDMBS-K019SARS-CoV-2 Spike NTD-coupled Magnetic Beads

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Spike S1MBS-K001SARS-CoV-2 Spike S1-coupled Magnetic Beads

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Spike S1MBS-K002SARS-CoV-2 Spike RBD-coupled Magnetic Beads

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Spike S2MBS-K018SARS-CoV-2 Spike S2-coupled Magnetic Beads

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Nucleocapsid proteinMBS-K017SARS-CoV-2 Nucleocapsid Protein-coupled Magnetic Beads

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ACE2MBS-K013Human ACE2-coupled Magnetic Beads

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BCMAMBS-K004Human BCMA-coupled Magnetic Beads

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CD19MBS-K005Human CD19-coupled Magnetic Beads

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CD3 epsilonMBS-K026Human CD3E-coupled Magnetic Beads

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CD38MBC-K010Human CD38-coupled Magnetic Beads

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CD3E & CD3DMBS-K003Human CD3E & CD3D Heterodimer-coupled Magnetic Beads

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CD73MBS-K022Human CD73-coupled Magnetic Beads

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EGF RMBE-K012Human EGFR-coupled Magnetic Beads

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EGFRvIIIMBS-K020Human EGFRvIII-coupled Magnetic Beads

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EpCAMMBE-K011Human EpCAM-coupled Magnetic Beads

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Her2MBS-K006Human HER2-coupled Magnetic Beads

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HGF RMBS-K021Human HGF R-coupled Magnetic Beads

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PSMAMBP-K007Human PSMA-coupled Magnetic Beads

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ROR1MBR-K009Human ROR1-coupled Magnetic Beads

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Siglec-3MBC-K008Human CD33-coupled Magnetic Beads

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StreptavidinSMB-B01Magnetic Beads™ Streptavidin

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Reference:

 1.Duchemin M, Tudor D,  Cottignies-Calamarte A, Bomsel M. Antibody-Dependent Cellular Phagocytosis of  HIV-1-Infected Cells Is Efficiently Triggered by IgA Targeting HIV-1 Envelope  Subunit gp41. Front Immunol. 2020 Jun 9;11:1141. doi: 10.3389. Retrieved from https://www.frontiersin.org/articles/10.3389/fimmu.2020.01141/full

 

  2.Lloyd, Y.M., Ngati, E.P., Salanti, A. et  al. A versatile, high through-put, bead-based phagocytosis assay  for Plasmodium falciparum . Sci Rep 7, 14705  (2017). Retrieved from https://doi.org/10.1038/s41598-017-13900-4

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