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Your Position: Home > CDPro CHO-2 Medium, Powder

BPM CDPro CHO-2 Medium is specifically designed and optimized for batch and fed-batch culture process development and production of recombinant CHO cells especially for CHO-S, CHO-K1, GS CHO cells in a chemically defined culture environment. CDPro CHO-2 medium is a well-balanced and rich medium, it can support higher cell density, longer process duration, better process yield as well as more compatible with downstream processing due to good cell health status at harvest. It is suitable for cell culture process of pilot and commercial scale production of antibody/protein.  CDPro CHO-2 is an animal origin-free, chemically defined medium that contains no proteins, polypeptides, hydrolysates, or components of unknown composition.

Product Features:
  • Designed for high density and yield of recombinant CHO cells culture process
  • >150% cell growth performance compared competitive alternatives
  • 2- 4 days longer process duration compared with industrial average
  • High viability at harvest to reduce complexity of downstream processing
  • Protein free, animal component free, chemically define and regulatory friendly
  • Extensive support by cell culture expert
Concentrated Cell Line Form Serum Endotoxin Glutamine Culture Type Phenol Red
1X CHO Powder Serum Free Low No Suspension Culture No
Product Classification Buffer system Regulatory Statement Shelf Life
Protein-Free, Chemically Defined;Serum-Free,   Animal Origin-Free Sodium Bicarbonate  For Research and Further Cell Culture Manufacturing Use Only 18 months
Catalog # Name of Product Product Size Storage Shipping
CM-1222-11 CDPro CHO-2 Medium 10L at +2-8℃, in dark RT
CM-1222-12 CDPro CHO-2 Medium 50L at +2-8℃, in dark RT
CM-1222-13 CDPro CHO-2 Medium 1000L at +2-8℃, in dark RT
Precaution:
  • CDPro CHO-2 requires supplementation of L-glutamine (4 - 8 mM) and sodium bicarbonate (2.3 g/L) for pH adjustment prior to use.     
  • CDPro CHO-2 is made without hypoxanthine and thymidine to support DHFR amplified systems. For other applications, HT supplementation is required prior to use
  • L-glutamine level must be considered in fed batch or perfusion model or your cells are sensitive to ammonia which is more likely generated in presence of high concentration of glutamine.
  • Formulated without phenol red to avoid estrogen-like effects of phenol red.
Cell Culture Conditions:

Standard cell culture conditions of growing CHO cells in CDPro CHO-2 medium are 36 to 38°C in a humidified air of 5-8% CO2. Under aseptic and cultivation conditions, cell culture vessel can be shaker flask, spinner flask and Wave or any other stirred bioreactors. Shaker flask volume can be from 125 mL to 1000 mL with approximate 20% to 30% working volume in suspension, and an orbital shaker platform rotating at 115 to 150 rpm is recommended. Loosen caps of flasks or use with vented caps for gas exchange is required for high density cell growth. Avoid overexposure of cultures to light.

Recommended Products:
Catalog # Product Name Descriptions
CM-1221 CDPro CHO-1 Medium Specially designed for transfected CHO cells
CM-1223 CDPro CHO-3 Medium Universal CHO medium
CM-1224 PFPro CHO-1 Medium Specially designed for DHFR deficient CHO cells
CM-1225 PFPro CHO-2 Medium Easy adaptation CHO medium(coming soon)
CF-1220 CD Feed CHO MAX-1 Concentrated supplement for fed batch process
CF-1221 CD Feed CHO MAX-2 Concentrated supplement for fed batch process

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