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Your Position: Home > FMC63

FMC63

FMC63 Protein Product ListCompare or Buy

Product> Filter Results

Cat. No.	Species	Product Description
FM3-S93	Rabbit	Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (recommended for ADA assay) (MALS verified)
FM3-BY54	Mouse	Biotinylated Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1, Avitag™ (Y45)
FM3-HPY53	Mouse	PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45)
FM3-Y45P1	Mouse	Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (HEK293)
FM3-FY45	Mouse	FITC-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45)
FM3-BY45	Mouse	Biotinylated Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45)
FM3-Y45	Mouse	Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45)

FMC63 Part of Bioactivity data

FM3-BY54-Cell-based assay

FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1, Avitag (Cat. No. FM3-BY54) and isotype control respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

Protocol

FM3-HPY53-Cell-based assay

1e6 of the Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-HPY53) and negative control antibody respectively, PE signal was used to evaluate the binding activity (QC tested).

Protocol

FM3-S93-MALS-HPLC

Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. ) MALS images

The purity of Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. FM3-S93) was more than 90% and the molecular weight of this protein is around 130-145 kDa verified by SEC-MALS.

Report

Bioactivity-ELISA

Immobilized FMC63 scFv at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. FM3-S93) with a linear range of 0.098-0.78 ng/mL (QC tested).

FM3-S93-ELISA

Immobilized FMC63 scFv at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. FM3-S93) with a linear range of 0.098-0.78 ng/mL (QC tested).

Protocol

FMC63 Molecule Synonym Name

Anti-FMC63 Ab & Anti-FMC63 scFv Antibody

FMC63 Molecule Background

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.