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DNP

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Part of Bioactivity data

DNP-BM190-Cell-based assay
FACS assay shows that recombinant Biotinylated FACS

2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Avitag Isotype Control (Cat. No. DNP-BM190) and positive control antibody (Cat. No. FM3-BY54) respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

DNP-BM190-Cell-based assay
FACS assay shows that recombinant Biotinylated FACS

5e5 of PBMCs were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Avitag Isotype Control (Cat. No. DNP-BM190) and isotype control antibody (Cat. No. FM3-BY54) respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

DNP-BM1-ELISA
Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype ControlBiotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-BM1) ELISA bioactivity

Measured by its binding ability in a functional ELISA. Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-BM1) with a linear range of 1-39 ng/mL (QC tested).

DNP-BM2-MALS-HPLC
Biotinylated Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (Cat. No. ) MALS images

The purity of Biotinylated Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (Cat. No. DNP-BM2) was more than 95% and the molecular weight of this protein is around 155-175 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Monoclonal Anti-DNP antibody, Human IgG1 Isotype ControlBiotinylated Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (Cat. No. DNP-BM2) ELISA bioactivity

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (Cat. No. DNP-BM2) with a linear range of 1-20 ng/mL (QC tested).

Synonym Name

DNP,Dinitrophenyl

Background

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

Clinical and Translational Updates

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