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Your Position: Home > Cytokines > VEGF165 > ActiveMax® Human VEGF165 Protein, Tag Free (HPLC-verified)

ActiveMax® Human VEGF165 Protein, Tag Free (HPLC-verified)

  • Synonym
    RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
  • Source
    ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (VE5-H4210) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # NP_001165097).
    Predicted N-terminus: Ala 27
  • Molecular Characterization
    Online(Ala 27 - Arg 191) NP_001165097

    This protein carries no "tag".

    The protein has a calculated MW of 19.0 kDa (monomer). As a result of glycosylation, the protein migrates as 24 kDa (monomer) under reducing (R) condition, and 43-50 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) SDS-PAGE gel

ActiveMax® Human VEGF165, Tag Free (HPLC-verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

SEC-HPLC
ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. ) HPLC images

A SEC-HPLC analysis showing 95% of ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (VE5-H4210) present as active homodimers.

Bioactivity-ELISA
ActiveMax® Human VEGF165, Tag Free (HPLC-verified)ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) ELISA bioactivity

Immobilized ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) at 0.01 μg/mL (100 μL/well) can bind Human VEGF R1, His Tag (Cat. No. VE1-H5220) with a linear range of 4-31 ng/mL (QC tested).

ActiveMax® Human VEGF165, Tag Free (HPLC-verified)ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) ELISA bioactivity

Immobilized ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R2, Avitag,His Tag (Cat. No. KDR-H82E5) with a linear range of 10-156 ng/mL (Routinely tested).

ActiveMax® Human VEGF165, Tag Free (HPLC-verified)ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) ELISA bioactivity

Immobilized ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Bevacizumab and 0.5 μg/mL (100 μL/well) Biotinylated Human VEGF R2, Avitag,His Tag (Cat. No. KDR-H82E5) with a half maximal inhibitory concentration (IC50) of 0.70 μg/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Avastin (Bevacizumab) captured on CM5 chip via ant-human IgG Fc antibodies surface, can bind ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 4.216-9.281 ng/ml (Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

Inhibition assay shows that the proliferation effect of ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) is inhibited by increasing concentration of anti-VEGF mAb (Avastin). The concentration of VEGF165 used is 20 ng/ml. The ED50 is 0.065-0.229 μg/mL (Routinely tested).

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  • Number of Launched Drugs:14 Details
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  • Latest Research Phase:Approved

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