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Your Position: Home > Bio-Markers & CD Antigens > Human-ErbB-2-HER2-Protein

Human Her2 / ErbB2 Protein

  • Synonym
    ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    Human Her2, His Tag (HE2-H5225) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # AAA75493).
    Predicted N-terminus: Thr 23
  • Molecular Characterization
    Her2(Thr 23 - Thr 652) AAA75493

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 70.2 kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Her2, His Tag (Cat. No. HE2-H5225) SDS-PAGE gel

Human Her2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human Her2, His TagHuman Her2, His Tag (Cat. No. HE2-H5225) ELISA bioactivity

Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 0.05 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.2-3 ng/mL (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized Herceptin on CM5 Chip via anti-human Fc IgG, can bind Human Her2 Protein (Cat. No. HE2-H5225) with an affinity constant of 0.147nM as determined in SPR assay (Biacore T200) ( Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Immobilized Herceptin on AHC Biosensor, can bind Human Her2 Protein (Cat. No. HE2-H5225) with an affinity constant of 0.93 nM as determined in BLI assay (Fortebio Octet 96) ( Routinely tested).

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Price(USD) : $290.00

Inventory : In stock

Price(USD) : $2180.00

Inventory : In stock

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