| ID | Components | Size |
|---|---|---|
| EP101-C01 | High-bind Plate | 1 plate |
| EP101-C02 | Human PD-L1 | 25 μg |
| EP101-C03 | Anti-PD-1 Neutralizing Antibody | 20 μg |
| EP101-C04 | Human PD-1-Biotin | 5 μg |
| EP101-C05 | Streptavidin-HRP | 10 μg |
| EP101-C06 | Coating Buffer | 12 mL |
| EP101-C07 | 10xWashing Buffer | 50 mL |
| EP101-C08 | Blocking Buffer | 50 mL |
| EP101-C09 | Substrate Solution | 12 mL |
| EP101-C10 | Stop Solution | 7 mL |
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human PD-L1.
b) Add your molecule of interest to the tests.
c) Add human PD-1-Biotin to bind the coated human PD-L1.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit PD-1 : PD-L1 binding will be determined by comparing OD readings among different experimental groups.
![PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay PairPD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair (Cat. No. EP-101) ELISA bioactivity](/static/main/products/images/elisa/EP-101-E1.jpg)
BINDING OF BIOTINYLATED HUMAN PD-1 TO IMMOBILIZED HUMAN PD-L1 IN A FUNCTIONAL ELISA ASSAY.
Immobilized human PD-L1 protein at 2 μg/mL (100 μL/well) can bind human PD-1-Biotin with a linear range of 0.01-0.2 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting (QC tested).
![PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay PairPD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair (Cat. No. EP-101) ELISA bioactivity](/static/main/products/images/elisa/EP-101-E2.jpg)
INHIBITION OF PD-1-PD-L1 BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY (CATALOG # EP101-C03) MEASURED USING THE PD-1 [BIOTINYLATED] : PD-L1 INHIBITOR SCREENING ELISA ASSAY PAIR (CATALOG # EP-101).
Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Price(USD) : $600.00
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