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Your Position: Home > Inhibitory Kit > PD-1 & PD-L1 > EP-101

PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair

For research use only.
Materials Provided
EP101-C01High-bind Plate1 plate
EP101-C02Human PD-L125 μg
EP101-C03Anti-PD-1 Neutralizing Antibody20 μg
EP101-C04Human PD-1-Biotin5 μg
EP101-C05Streptavidin-HRP10 μg
EP101-C06Coating Buffer12 mL
EP101-C0710xWashing Buffer 50 mL
EP101-C08Blocking Buffer50 mL
EP101-C09Substrate Solution12 mL
EP101-C10Stop Solution7 mL
  • Background
    Immune checkpoint pathway is a focal point of today’s cancer research. PD-1 is one of the best characterized checkpoint proteins. The binding between PD-1 and its ligand PD-L1 suppresses T-cell activation and allows cancer cells to escape from body’s immune surveillance. Therefore, the pharmaceutical inhibition of PD-1 or its ligand has been considered a promising strategy by many oncologists.
  • Application
    This kit is developed for screening for inhibitors of human PD-1 binding to human PD-L1.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new PD-1 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human PD-1 to immobilized human PD-L1 in a functional ELISA assay, and employs a simple colorimetric sandwich ELISA platform.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human PD-L1.

    b) Add your molecule of interest to the tests.

    c) Add human PD-1-Biotin to bind the coated human PD-L1.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the ability of your compound to inhibit PD-1 : PD-L1 binding will be determined by comparing OD readings among different experimental groups.

Typical Data
PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay PairPD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair (Cat. No. EP-101) ELISA bioactivity

Immobilized human PD-L1 protein at 2 μg/mL (100 μL/well) can bind human PD-1-Biotin with a linear range of 0.01-0.2 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting (QC tested).

PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay PairPD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair (Cat. No. EP-101) ELISA bioactivity

Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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