|EP101-C01||High-bind Plate||1 plate|
|EP101-C02||Human PD-L1||25 μg|
|EP101-C03||Anti-PD-1 Neutralizing Antibody||20 μg|
|EP101-C04||Human PD-1-Biotin||5 μg|
|EP101-C06||Coating Buffer||12 mL|
|EP101-C07||10xWashing Buffer||50 mL|
|EP101-C08||Blocking Buffer||50 mL|
|EP101-C09||Substrate Solution||12 mL|
|EP101-C10||Stop Solution||7 mL|
The unopened kit should be stored at 2°C to 8°C. The shelf life is 12 months from the date of receipt. The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human PD-L1.
b) Add your molecule of interest to the tests.
c) Add human PD-1-Biotin to bind the coated human PD-L1.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit PD-1 : PD-L1 binding will be determined by comparing OD readings among different experimental groups.
BINDING OF BIOTINYLATED HUMAN PD-1 TO IMMOBILIZED HUMAN PD-L1 IN A FUNCTIONAL ELISA ASSAY.
Immobilized human PD-L1 protein at 2 μg/mL (100 μL/well) can bind human PD-1-Biotin with a linear range of 0.01-0.2 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting. (QC tested)
INHIBITION OF PD-1-PD-L1 BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY (CATALOG # EP101-C03) MEASURED USING THE PD-1 [BIOTINYLATED] : PD-L1 INHIBITOR SCREENING ELISA ASSAY PAIR (CATALOG # EP-101).
Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting. (QC tested)
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