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Your Position: Home > ActiveMax® Cytokines > Human PDGF-BB Protein,HEK293 expressed,ActiveMax

ActiveMax® Recombinant Human PDGF-BB

  • Synonym
    PDGFBB,PDGFB,FLJ12858,PDGF2,SIS,SSV,c-sis
  • Source
    ActiveMax® Human PDGF-BB (PDB-H4219) is expressed from human 293 cells (HEK293). It contains AA Ser 82 - Thr 190 (Accession # AAH29822).
  • Molecular Characterization
    PDGF-B(Ser 82 - Thr 190) AAH29822

    rhPDGF-BB is a disulfide-linked homodimeric polypeptide chain containing 2×109 amino acids, and has a calculated MW of 25 kDa. The reducing (R) protein migrates as a 15 kDa polypeptide and the non-reducing (NR) disulfide-linked homodimer migrates as a 28 kDa protein.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reducing (R) and non-reducing (NR) SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ActiveMax® Human PDGF-BB (Cat. No. PDB-H4219) SDS-PAGE gel

ActiveMax® Human PDGF-BB on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
ActiveMax® Human PDGF-BBActiveMax® Human PDGF-BB (Cat. No. PDB-H4219) ELISA bioactivity

Immobilized ActiveMax® Human PDGF-BB (Cat. No. PDB-H4219) at 5 µg/mL (100 µl/well) can bind Human PDGF R beta, Fc Tag (Cat. No. PDB-H5252) with a linear range of 0.02-0.6 µg/mL (QC tested).

ActiveMax® Human PDGF-BBActiveMax® Human PDGF-BB (Cat. No. PDB-H4219) ELISA bioactivity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. The ED50 was 0.5-20 ng/mL (Routinely tested).

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Price(USD) : $198.00

Inventory : In stock

Price(USD) : $350.00

Inventory : In stock

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