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Recombinant HIV-1 (CN54) GP120

  • Synonym
  • Source
    Recombinant HIV-1 GP120 Protein (HIV-1 /Clade B/C (CN54)) (Recombinant HIV-1 GP120 (CN54)) derived from the env. gene of HIV-1 strain CN54 gp160 (GenBank Gene Ac. # AX149771, Thr27-Arg498) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
    Predicted N-terminus: Thr 27
  • Molecular Characterization
    GP120 (HIV)(Thr 27 - Arg 498) Gene Num.: AX149771

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 53.8 kDa. The protein migrates as 80-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
HIV-1 (CN54) GP120 (Cat. No. GP4-V15227) SDS-PAGE gel

HIV-1 (CN54) GP120 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HIV-1 (CN54) GP120HIV-1 (CN54) GP120 (Cat. No. GP4-V15227) ELISA bioactivity

Immobilized HIV GP120 (HIV), His Tag (Cat. No. GP4-V15227) at 5μg/mL (100 μL/well) can bind Human CD4, Fc Tag (Cat. No. CD4-H5259)) with a linear range of 4.9-78 ng/mL (QC tested).

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