This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.2 kDa. The protein migrates as 29-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Pre-activation is required for enzymatic assays. Please dilute Human Kallikrein 7 to 200 µg/mL in TCNB buffer (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5), and then dilute Thermolysin to a final concentration of 20 μg/mL in TCNB buffer. Combine equal volume of diluted KLK7 and Thermolysin together and incubate at 37 ℃ for 2 hours. Then stop reaction with equal volume of 100 mM EDTA diluted in 50 mM Tris, 150 mM NaCl, pH 8.5. Please note that the optimal treatment time may need to be determined empirically.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Human Kallikrein 7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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