This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 60.8 kDa. The protein migrates as 80 - 90 kDa and 35 kDa Fc fragment under reducing (R) condition (SDS-PAGE) due to glycosylation and auto-cleavage.
>60% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human RAGE, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 60%.
Authors: Sen Zhang, et al.
Journal: Am J Transl Res 2017
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high quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
The affinity constant of SARS-CoV-2 S protein (active trimer) binding to human ACE2 protein was 35.3 nM as verified by SPR.
The affinity constant of human ACE2 protein binding to SARS-CoV-2 S1 protein was 16.7 nM as verified by SPR. Human ACE2 proteins was verified by SEC-MALS and bioactivity was verified by SPR, BLI and ELISA. Protocols are offered for free.
Binding signal of biotinylated human ACE2 and SARS-CoV-2 S protein RBD was inhibited by SARS-CoV-2 inhibitor with the IC50 10.69 nM.
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