This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 60.8 kDa. The protein migrates as 80 - 90 kDa and 35 kDa Fc fragment under reducing (R) condition (SDS-PAGE) due to glycosylation and auto-cleavage.
>60% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Human RAGE, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 60%.
Authors: Sen Zhang, et al.
Journal: Am J Transl Res 2017
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