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Your Position: Home > All Other Proteins > RAGE > Human AGER /RAGE Protein,C-Fc Tag

Human RAGE / AGER Protein, Fc Tag

  • Synonym
    AGER,RAGE
  • Source
    Human RAGE, Fc Tag (AGR-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Ala 344 (Accession # NP_001127).
    Predicted N-terminus: Ala 23
  • Molecular Characterization
    RAGE(Ala 23 - Ala 344) NP_001127

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 60.8 kDa. The protein migrates as 80 - 90 kDa and 35 kDa Fc fragment under reducing (R) condition (SDS-PAGE) due to glycosylation and auto-cleavage.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >60% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human RAGE, Fc Tag (Cat. No. AGR-H5253) SDS-PAGE gel

Human RAGE, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 60%.

Bioactivity
Human RAGE, Fc TagHuman RAGE, Fc Tag (Cat. No. AGR-H5253) ELISA bioactivity

Immobilized Human RAGE, Fc Tag (Cat. No. AGR-H5253) at 20 μg/mL (100 μL/well) can bind Human HMGB1, His Tag (Cat. No. HM1-H5220) with a linear range of 0.313-5 μg/mL (QC tested).

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