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Your Position: Home > Immune Checkpoint Proteins > PD-1 > Biotinylated Human PDCD1 Protein, C-Fc, His & AVI Tagged,Super Sensitivity

Biotinylated Human PD-1 / PDCD1, His Tag & Fc Tag, Avi Tag (Avitag™)

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    MABSol® Biotinylated Human PD-1, His Tag, Fc Tag (PD1-H82F2) is expressed from human HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # NP_005009).
    N-terminal Sequence Analysis: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Gln 167) NP_005009

    This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc and his tags. The protein has a calculated MW of 44.4 kDa. The protein migrates as 50-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100-130 kDa under non-reducing (NR) condition.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human PD-1, His Tag, Fc Tag (Cat. No. PD1-H82F2) SDS-PAGE gel

Biotinylated Human PD-1, His Tag, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human PD-1, His Tag, Fc TagBiotinylated Human PD-1, His Tag, Fc Tag (Cat. No. PD1-H82F2) ELISA bioactivity

Immobilized Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at 5 μg/mL (100 µl/well) can bind Biotinylated Human PD-1, His Tag, Fc Tag (Cat. No. PD1-H82F2) with a linear range of 0.01-0.1 μg/mL (QC tested).

Biotinylated Human PD-1, His Tag, Fc TagBiotinylated Human PD-1, His Tag, Fc Tag (Cat. No. PD1-H82F2) ELISA bioactivity

Immobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, His Tag, Fc Tag (Cat. No. PD1-H82F2) with a linear range of 0.4-6 ng/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

Flow Cytometry assay shows that recombinant Biotinylated Human PD-1, His Tag, Fc tag (Cat No. PD1-H82F2) can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 μg/ml (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of Biotinylated Human PD-1, His Tag, Fc tag to 293 overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-PD-L1 antibody. The concentration of PD1 used is 1μg/ml. The IC50 is 0.362 μg/ml (Routinely tested).

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