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>Human CD3 epsilon & CD3 delta Heterodimer Protein, Fc Tag
Human CD3 epsilon & CD3 delta Heterodimer Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 39.2 kDa (CD3E) and 37.5 kDa (CD3D) . Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and subunit CD3D is fused with flag-tagged human IgG1 Fc fragment at the C-terminus. The reducing (R) heterodimer protein migrates as 40-55 kDa due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human CD3E & CD3D, His Tag, Fc Tag, Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human CD3E & CD3D, His Tag, Fc Tag, Flag Tag (Cat. No. CDD-H52W0) at 2 μg/mL (100 μL/well)can bind OKT3 (mouse IgG2a) (Cat. No. CDE-M120a) with a linear range of 10-78 ng/mL (QC tested).
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Price(USD) : $319.00
Price(USD) : $1936.00
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