This protein carries a twin strep tag at the C-terminus.
The protein has a calculated MW of 73.5 kDa. The protein migrates as 17 kDa and 62-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.
>97% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human PCSK9, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.
Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μl/well) can bind Human PCSK9, Strep Tag (Cat. No. PC9-H528c ) with a linear range of 4-200 ng/mL (QC tested).
FACS analysis shows that Human PCSK9, Strep Tag (Cat. No. PC9-H528c) inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 0.6923-3.589 μg/mL.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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