Human PCSK9 Protein, Strep Tag

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
Source
Human PCSK9, Strep Tag (PC9-H528c) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # AAI66619).
Predicted N-terminus: Gln 31
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Molecular Characterization
This protein carries a twin strep tag at the C-terminus.
The protein has a calculated MW of 73.5 kDa. The protein migrates as 17 kDa and 62-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>97% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human PCSK9, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

Bioactivity-ELISA

Human PCSK9, Strep TagHuman PCSK9, Strep Tag (Cat. No. PC9-H528c) ELISA bioactivity

Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μl/well) can bind Human PCSK9, Strep Tag (Cat. No. PC9-H528c ) with a linear range of 4-200 ng/mL (QC tested).

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Background
Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
References
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