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Your Position: Home > All Other Proteins > Siglec-2 > Human CD22 /Siglec-2 (176-687) Protein

Human Siglec-2 / CD22 (176-687) Protein

  • Synonym
    CD22,SIGLEC2,BL-CAM,SIGLEC-2,Siglec2,SIGLEC2FLJ22814
  • Source
    Human Siglec-2 (176-687), His Tag (SI2-H5228) is expressed from human 293 cells (HEK293). It contains AA Trp 176 - Arg 687 (Accession # P20273-1).
    Predicted N-terminus: Trp 176
  • Molecular Characterization
    Online(Trp 176 - Arg 687) P20273-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 57.8 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-2 (176-687), His Tag (Cat. No. SI2-H5228) SDS-PAGE gel

Human Siglec-2 (176-687), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Siglec-2 (176-687), His TagHuman Siglec-2 (176-687), His Tag (Cat. No. SI2-H5228) ELISA bioactivity

Immobilized Human Siglec-2 (176-687), His Tag (Cat. No. SI2-H5228) at 2 μg/mL (100 μL/well) can bind Anti-Human CD22 MAb, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-Human CD22 MAb (human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human Siglec-2 (176-687), His Tag (Cat. No. SI2-H5228) with an affinity constant of 11.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Evaluation of CAR expression
FACS Analysis of Anti-CD22 CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Siglec-2 (176-687), His Tag (Cat. No. SI2-H5228, 3 μg/ml) and C. His Tag Protein Control, followed by FITC Anti-6xHis Tag Antibody. A. Non-transfected 293 cells and C. His Tag Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD22-scFv) expression and FITC was used to evaluate the binding activity of Human Siglec-2 (176-687), His Tag (Cat. No. SI2-H5228).

  • Background
    B-cell receptor CD22 is also known as Sialic acid-binding Ig-like lectin 2 (Siglec-2), B-lymphocyte cell adhesion molecule (BL-CAM), T-cell surface antigen Leu-14, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. CD22 mediates B-cell B-cell interactions, and may be involved in the localization of B-cells in lymphoid tissues. Siglec-2 / CD22 binds sialylated glycoproteins, one of which is CD45. Siglec2 / CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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