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Your Position: Home > All Other Proteins > PCSK9 > Biotinylated Human PCSK9 Protein, Avitag,His Tag

Biotinylated Human PCSK9 Protein, Avitag™,His Tag

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    MABSol® Biotinylated Human PCSK9, Avitag,His Tag (PC9-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # AAI66619).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    Online(Gln 31 - Gln 692) AAI66619

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 72.0 kDa. The protein migrates as 20 kDa and 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) SDS-PAGE gel

Biotinylated Human PCSK9, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA
Biotinylated Human PCSK9, Avitag,His TagBiotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) ELISA bioactivity

Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) with a linear range of 2-31 ng/mL (QC tested).

Biotinylated Human PCSK9, Avitag,His TagBiotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) ELISA bioactivity

Immobilized Biotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) can bind PCSK9 Mab, Human IgG with a linear range of  0.05-1.5 ng/mL. The biotinylated PCSK9 is coated at 1 μg/mL (100 μL/well) on a streptavidin coated plates (Routinely tested).

Biotinylated Human PCSK9, Avitag,His TagBiotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) ELISA bioactivity

Immobilized Human VLDL R, His Tag (Cat. No. VLR-H5227) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) with a linear range of 10-156 ng/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that Biotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 1.502-2.378 μg/mL.

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Price(USD) : $350.00

Price(USD) : $1380.00

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