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Your Position: Home > All Other Proteins > Fas > Human-Fas-CD95-Protein-C-Fc Tag

Human Fas / TNFRSF6 / CD95 Protein, Fc Tag

  • Synonym
    FAS,ALPS1A,APO1,APT1,CD95,FAS1,FASTM,TNFRSF6,FasR
  • Source
    Human Fas, Fc Tag (FAS-H5252) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Asn 173 (Accession # AAH12479.1).
    Predicted N-terminus: Gln 26
  • Molecular Characterization
    Online(Gln 26 - Asn 173) AAH12479.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 42.8 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose  is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human Fas, Fc Tag (Cat. No. FAS-H5252) SDS-PAGE gel

Human Fas, Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Fas, Fc TagHuman Fas, Fc Tag (Cat. No. FAS-H5252) ELISA bioactivity

Immobilized Human Fas Ligand, His Tag (Cat. No. FAL-H5241) at  2 μg/mL (100 μL/well)can bind Human Fas, Fc Tag (Cat. No. FAS-H5252) with a linear range of 4-31 ng/mL (QC tested).

  • Background
    The Fas is also known as FAS receptor (FasR), apoptosis antigen 1 (APO-1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6). is a death receptor on the surface of cells that leads to programmed cell death (apoptosis). It is one of two apoptosis pathways, the other being the mitochondrial pathway. FasR is located on chromosome 10 in humans and 19 in mice. Similar sequences related by evolution (orthologs) are found in most mammals. Fas forms the death-inducing signaling complex (DISC) upon ligand binding. Membrane-anchored Fas ligand trimer on the surface of an adjacent cell causes trimerization of Fas receptor. This event is also mimicked by binding of an agonistic Fas antibody, though some evidence suggests that the apoptotic signal induced by the antibody is unreliable in the study of Fas signaling. To this end, several clever ways of trimerizing the antibody for in vitro research have been employed.Upon ensuing death domain (DD) aggregation, the receptor complex is internalized via the cellular endosomal machinery. This allows the adaptor molecule FADD to bind the death domain of Fas through its own death domain. Recently, Fas has also been shown to promote tumor growth, since during tumor progression, it is frequently downregulated or cells are rendered apoptosis resistant. Cancer cells in general, regardless of their Fas apoptosis sensitivity, depend on constitutive activity of Fas. This is stimulated by cancer-produced Fas ligand for optimal growth.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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