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Your Position: Home > All Other Proteins > Carboxypeptidase M > Human CPM /Carboxypeptidase M Protein

Human Carboxypeptidase M (CPM) Protein

  • Synonym
    CPM,Carboxypeptidase M
  • Source
    Human Carboxypeptidase M, His Tag (CPM-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 18 - His 422 (Accession # AAH22276).
    Predicted N-terminus: Leu 18
  • Molecular Characterization
    CPM(Leu 18 - His 422) AAH22276

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 47.1 kDa. The protein migrates as 49 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM MES, 100 mM NaCl, pH6.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Carboxypeptidase M, His Tag (Cat. No. CPM-H5222) SDS-PAGE gel

Human Carboxypeptidase M, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    Carboxypeptidase M (CPM) belongs to the peptidase M14 family. CPM specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins, and the activity is inhibited by O-phenanthroline and MGTA and activated by cobalt. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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