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Your Position: Home > All Other Proteins > CD3 epsilon > Cynomolgus CD3E /CD3 Epsilon Protein, C-Fc Tag

Cynomolgus CD3 epsilon Protein, His Tag & Fc Tag

  • Synonym
  • Source
    Cynomolgus CD3 epsilon, His Tag & Fc Tag (CDE-C5254) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    CD3E(Gln 22 - Asp 117) Q95LI5

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 38.3 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

Cynomolgus CD3 epsilon, His Tag & Fc Tag (Cat. No. CDE-C5254) SDS-PAGE gel

Cynomolgus CD3 epsilon, His Tag & Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Cynomolgus CD3 epsilon, His Tag & Fc TagCynomolgus CD3 epsilon, His Tag & Fc Tag (Cat. No. CDE-C5254) ELISA bioactivity

Immobilized SP34-2 at 2 μg/mL (100 μL/well) can bind Cynomolgus CD3E, His Tag, Fc Tag (Cat. No. CDE-C5254) with a linear range of 0.2-3 ng/mL (Routinely tested).

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