0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Fc Receptor Proteins > Fc gamma RI / CD64 > CD4-M5227

Mouse Fc gamma RI / CD64 Protein, His Tag (BLI verified)

  • Synonym
    FCGR1A,FCG1,FCGR1,IGFR1,CD64,CD64A,FCRI
  • Source
    Mouse CD64, His Tag (CD4-M5227) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Pro 297 (Accession # P26151-1).
    Predicted N-terminus: Glu 25
  • Molecular Characterization
    Online(Glu 25 - Pro 297) P26151-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 32.3 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse CD64, His Tag (Cat. No. CD4-M5227) SDS-PAGE gel

Mouse CD64, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse CD64, His TagMouse CD64, His Tag (Cat. No. CD4-M5227) ELISA bioactivity

Immobilized Anti-CD3 epsilon MAb, Mouse IgG2a (Cat. No. CDE-M120a) at 2 μg/mL (100 μL/well) can bind Mouse CD64, His Tag (Cat. No. CD4-M5227) with a linear range of 1-8 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Captured Mouse CD64, His Tag (Cat. No. CD4-M5227) on NTA-Series S sensor chip can bind Herceptin® (Trastuzumab) with an affinity constant of 63.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Herceptin on Protein A Biosensor, can bind Mouse CD64, His Tag (Cat. No. CD4-M5227) with an affinity constant of 31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Comments (0)

Order Details

Price(USD) : $220.00

Price(USD) : $280.00

Price(USD) : $1800.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop