This protein carries a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 48.7 kDa. The predicted N-terminus is Gln 21. The reducing (R) protein migrates as 76 kDa in SDS-PAGE due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Human CD44, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD44 Protein, Fc Tag (HPLC-verified) (Cat.# PG1-H5255) was greater than 90% as determined by SEC-HPLC.
Authors: Bhattacharya D, et al.
Journal: J MATER CHEM B 2017
Application: Fluorescence polarization assay
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