This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.7 kDa. The protein migrates as 76 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human CD44, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) was greater than 90% as determined by SEC-HPLC.
Immobilized Hyaluronic Acid at 100 μg/mL (100 μL/well) can bind Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) with a linear range of 0.156-2.5 μg/mL (Routinely tested).
Authors: Bhattacharya D, et al.
Journal: J MATER CHEM B 2017
Application: Fluorescence polarization assay
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