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Your Position: Home > All Other Proteins > CD44 > PG1-H5255

Human CD44 Protein, Fc Tag (HPLC-verified)

  • Synonym
    CD44,CDW44,CSPG8,ECMR-III,HCELL,HUTCH-I,IN,LHR,MC56,MDU2,MDU3,MIC4,Pgp1,Epican
  • Source
    Human CD44, Fc Tag (HPLC-verified) (PG1-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Pro 220 (Accession # AAH04372).
    Predicted N-terminus: Gln 21
  • Molecular Characterization
    Online(Gln 21 - Pro 220) AAH04372

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 48.7 kDa. The protein migrates as 76 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) SDS-PAGE gel

Human CD44, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human CD44, Fc Tag (HPLC-verified) (Cat. No. ) HPLC images

The purity of Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
Human CD44, Fc Tag (HPLC-verified)Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) ELISA bioactivity

Immobilized Hyaluronic Acid at 100 μg/mL (100 μL/well) can bind Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) with a linear range of 0.156-2.5 μg/mL (Routinely tested).

Human CD44, Fc Tag (HPLC-verified)Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) ELISA bioactivity

Immobilized Human CD44, Fc Tag (HPLC-verified) (Cat. No. PG1-H5255) at 5 μg/mL (100 μL/well) can bind Hyaluronan biotin sodium salt with a linear range of 10-156 ng/mL (Routinely tested).

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