This is the new website for acrobiosystems.com. We would love to hear what you think of it - please send feedback

Welcome to ACROBiosystems

0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Bio-Markers & CD Antigens > Cynomolgus CD3E & CD3D Heterodimer

Cynomolgus CD3 epsilon & CD3 delta Heterodimer Protein, His Tag & Flag Tag

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    Recombinant Cynomolgus CD3E & CD3D heterodimer protein (Cynomolgus CD3E & CD3D heterodimer) Gln 22 - Asp 117 (CD3E,Q95LI5) & Phe 22 - Ala 105 (CD3D,Q95LI8) was produced in human 293 cells (HEK293) at ACROBiosystems.
    Predicted N-terminus: Gln 22 (CD3E) and Phe 22 (CD3D)
  • Molecular Characterization
    CD3E & CD3D(Gln 22 - Asp 117 (CD3E) and Phe 22 - Ala 105 (CD3D)) Q95LI5 (CD3E) and Q95LI8 (CD3D)

    The heterodimer Cynomolgus CD3E & CD3D heterodimer is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) and Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 19-22 kDa and 25-40 kDa due to glyosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus CD3E & CD3D, His Tag & Flag Tag (Cat. No. CDD-C52W4) SDS-PAGE gel

Cynomolgus CD3E & CD3D, His Tag & Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity
Cynomolgus CD3E & CD3D, His Tag & Flag TagCynomolgus CD3E & CD3D, His Tag & Flag Tag (Cat. No. CDD-C52W4) ELISA bioactivity

Immobilized SP34-2 at 5 μg/mL (100 μL/well) can bind Cynomolgus CD3E & CD3D, His Tag, Flag Tag (Cat. No. CDD-C52W4) with a linear range of 0.6-5 ng/mL (Routinely tested).

Comments (0)

Order Details

Price(USD) : $315.00

Inventory : In stock

Price(USD) : $1980.00

Inventory : In stock

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Related Products

Questions & Comments

This web search service is supported by Google Inc.