This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.1 kDa. The protein migrates as 37-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus / Rhesus macaque PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Anti-Human PD-L1 MAb captured on CM5 chip via anti-human IgG Fc antibody surface, can bind Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) with an affinity constant of 23.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) with an affinity constant of 19.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Cynomolgus PD-1, Fc Tag (Cat. No.PD1-C5254) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) with an affinity constant of 1.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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