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Your Position: Home > All Other Proteins > Activin RIIA > Human ACVR2A /ACVR2 Protein,C-Fc Tag

Human Activin RIIA / ACVR2A Protein, Fc Tag

  • Synonym
    Activin receptor type IIA,ACTRIIA
  • Source
    Human Activin RIIA, Fc Tag (ACA-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 134 (Accession # NP_001607).
    Predicted N-terminus: Ala 20
  • Molecular Characterization
    ACVR2A(Ala 20 - Pro 134) NP_001607

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Activin RIIA, Fc Tag (Cat. No. ACA-H5253) SDS-PAGE gel

Human Activin RIIA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human Activin RIIA, Fc TagHuman Activin RIIA, Fc Tag (Cat. No. ACA-H5253) ELISA bioactivity

Immobilized Human Activin A, Tag Free (Cat. No. ACA-H421b) at 2 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. ACA-H5253) with a linear range of 0.08-1.25 μg/mL (QC tested).

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