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Your Position: Home > All Other Proteins > PCSK9 > Biotinylated Human PCSK9 (D374Y) Protein, C-His tagged Avi-tag

Biotinylated Human PCSK9 (D374Y), Avi Tag (Avitag™)

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    MABSol® Biotinylated Human PCSK9 (D374Y) (PCY-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7 (D374Y)).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    Online(Gln 31 - Gln 692) Q8NBP7 (D374Y)

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 73.7 kDa. The protein migrates as 16 kDa,18 kDa and 65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion.

    The D374Y mutation results in higher affinity of PCSK9 for LDLR.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human PCSK9, His Tag (Cat. No. PCY-H82E7) SDS-PAGE gel

Biotinylated Human PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human PCSK9, His TagBiotinylated Human PCSK9, His Tag (Cat. No. PCY-H82E7) ELISA bioactivity

Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μl/well) can bind Biotinylated Human (D374Y) PCSK9 (Cat. No. PCY-H82E7 ) with a linear range of 0.02-0.5 μg/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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