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Your Position: Home > All Other Proteins > Neuropilin-1 > Human NRP1 /Neuropilin‑1 /CD304 Protein,C-Fc Tag

Human Neuropilin-1 / NRP1 / CD304 Protein, Fc Tag

  • Synonym
    NRP1,Neuropilin-1,NRP,VEGF165R,CD304
  • Source
    Human Neuropilin-1, Fc Tag (NR1-H5252) is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Lys 644 (Accession # AAH07533).
    Predicted N-terminus: Phe 22
  • Molecular Characterization
    NRP1(Phe 22 - Lys 644) AAH07533

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 96.5 kDa. The protein migrates as 110-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human Neuropilin-1, Fc Tag (Cat. No. NR1-H5252) SDS-PAGE gel

Human Neuropilin-1, Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human Neuropilin-1, Fc TagHuman Neuropilin-1, Fc Tag (Cat. No. NR1-H5252) ELISA bioactivity

Immobilized Human VEGF165, Tag Free (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Human NRP1, Fc Tag (Cat. No. NR1-H5252) with a linear range of 0.4-6 ng/mL (QC tested).

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