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Your Position: Home > Recombinant Proteins > IL-12B & IL-12A > Human IL-12B & IL-12A Protein, His Tag & Flag Tag

Human IL-12B & IL-12A Heterodimer Protein, His Tag & Flag Tag

  • Synonym
    IL12,p70,Interleukin-12
  • Source
    Human IL-12B & IL-12A, His Tag & Flag Tag (IL2-H4210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) and Arg 23 - Ser 219 (IL-12A) (Accession # P29460-1 (IL12B) and P29459-1 (IL12A)).
    Predicted N-terminus: Ile 23 (IL12B) and Arg 23 (IL12A)
  • Molecular Characterization
    IL-12B & IL-12A(Ile 23 - Ser 328 (IL-12B) and Arg 23 - Ser 219 (IL-12A)) P29460-1 (IL12B) and P29459-1 (IL12A)

    The heterodimer Human IL-12B & IL-12A is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 36.6 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with polyhistidine tag at the C-terminus and subunit IL-12A is fused with flag tag at the C-terminus. The predicted N-terminus is Ile 23 (IL12B) and Arg 23 (IL12A). The reducing (R) heterodimer protein migrates as 43 kDa and 45 kDa (IL12B) and 35-40 kDa (IL12A) respectively due to glyosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-12B & IL-12A, His Tag & Flag Tag (Cat. No. IL2-H4210) SDS-PAGE gel

Human IL-12B & IL-12A, His Tag & Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human IL-12B & IL-12A, His Tag & Flag TagHuman IL-12B & IL-12A, His Tag & Flag Tag (Cat. No. IL2-H4210) ELISA bioactivity

Immobilized Human IL-12B & IL-12A, His Tag & Flag Tag (Cat. No. IL2-H4210) at 2 μg/mL (100 μL/well) can bind Anti IL23A & IL12B P40 domain with a linear range of 0.1-2 ng/mL (QC tested).

  • Background
    Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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