Rhesus macaque DDR2 Protein

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
DDR2,CD167b,NTRKR3,TKT,TYRO10
Source
Rhesus macaque DDR2, His Tag (DD2-C52H5) is expressed from human 293 cells (HEK293). It contains AA Lys 22 - Arg 399 (Accession # G7MEB8-1).
Predicted N-terminus: Lys 22
Request for sequence
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 44.6 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Rhesus macaque DDR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Background
Discoidin domain-containing receptor 2 (DDR2) is also known as CD167 antigen-like family member B (CD167b), Discoidin domain-containing receptor tyrosine kinase 2, Neurotrophic tyrosine kinase, receptor-related 3(NTRKR3), Tyrosine-protein kinase TYRO10, Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. DDR2 is required for normal bone development. DDR2 regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. DDR2 regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. DDR2 promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.
References
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