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Your Position: Home > Cytokines > FGF basic > Human bFGF /FGF basic Protein, Biotinylated

Biotinylated Human FGF basic, epitope tag free, ultra sensitivity (primary amine labeling)

  • Synonym
    FGF2,BFGF,FGFB,FGF basic,HBGF-2
  • Source
    MABSol® Biotinylated Human FGF basic (BFF-H8117) is expressed from E.coli cells. It contains AA Pro 143 - Ser 288 (Accession # NP_001997). It is the biotinylated form of Human FGF basic Protein, Tag Free (Cat # BFF-H4117).
    Predicted N-terminus: Met
  • Molecular Characterization
    FGF basic(Pro 143 - Ser 288) NP_001997

    The product does not contain any epitope tags. The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa on a SDS-PAGE gel under reducing (R) condition.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 4.5-5.5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human FGF basic (Cat. No. BFF-H8117) SDS-PAGE gel

Biotinylated Human FGF basic on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

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Price(USD) : $350.00

Price(USD) : $1250.00

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