Biotinylated Human FGF basic, epitope tag free, ultra sensitivity (primary amine labeling)

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
FGF2,BFGF,FGFB,FGF basic,HBGF-2
Source
MABSol® Biotinylated Human FGF basic (BFF-H8117) is expressed from E.coli cells. It contains AA Pro 143 - Ser 288 (Accession # NP_001997). It is the biotinylated form of Human FGF basic Protein, Tag Free (Cat # BFF-H4117).
Predicted N-terminus: Met
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Molecular Characterization
The product does not contain any epitope tags. The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa on a SDS-PAGE gel under reducing (R) condition.
Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Biotin:Protein Ratio
The biotin to protein ratio is 4.5-5.5 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by reduced SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Biotinylated Human FGF basic on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Citations
- A heparan sulfate microarray reveals that heparan sulfate-protein binding exhibit different ligand requirements
  Authors: Zong C, et al.
  Journal: J Am Chem Soc 2017
  Application: Cell-based ELISA
  Request for Full-text
Background
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. bFGF is a critical component of human embryonic stem cell culture medium. bFGF is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.
References
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