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Your Position: Home > All Other Proteins > CD4 > Rhesus macaque CD4 /LEU3 Protein,C-Fc Tag

Rhesus macaque CD4 Protein, Fc Tag

  • Synonym
    CD4,CD4mut,LEU3
  • Source
    Rhesus macaque CD4, Fc Tag (CD4-C5256) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # G7N5T8).
    Predicted N-terminus: Lys 26
  • Molecular Characterization
    CD4(Lys 26 - Trp 390) G7N5T8

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 67 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Rhesus macaque CD4, Fc Tag (Cat. No. CD4-C5256) SDS-PAGE gel

Rhesus macaque CD4, Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Rhesus macaque CD4, Fc TagRhesus macaque CD4, Fc Tag (Cat. No. CD4-C5256) ELISA bioactivity

Immobilized HIV GP120 (HIV), His Tag (Cat. No. GP5-V15224) at 10μg/mL (100 μL/well) can bind Rhesus macaque CD4, Fc Tag (Cat. No. CD4-C5256)) with a linear range of 0.04-0.31 μg/mL (QC tested).

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