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Your Position: Home > Cytokines > EGF > Human EGF Protein, Tag Free

Human EGF Protein, Tag Free

  • Synonym
  • Source
    Human EGF, Tag Free (EGF-H4114) is expressed from E.coli cells. It contains AA Asn 971 - Arg 1023 (Accession # P01133).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Asn 971 - Arg 1023) P01133

    This protein carries no "tag".

    The protein has a calculated MW of 6.2 kDa. The protein migrates as 7 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Human EGF, Tag Free (Cat. No. EGF-H4114) SDS-PAGE gel

Human EGF, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Human EGF, Tag FreeHuman EGF, Tag Free (Cat. No. EGF-H4114) ELISA bioactivity

Immobilized Human EGF, Tag Free (Cat. No. EGF-H4114) at 10 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His Tag, Avi Tag (Cat. No. EGR-H82E3) with a linear range of 0.156-5 μg/mL (QC tested).

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