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Your Position: Home > All Other Proteins > Recombinant Rat PCSK9 Protein

Rat PCSK9 Protein

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    Rat PCSK9, His Tag (PC9-R52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 691 (Accession # P59996-1).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    PCSK9(Gln 31 - Gln 691) P59996-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 73.2 kDa. The protein migrates as 20 kDa and 63-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat PCSK9, His Tag (Cat. No. PC9-R52H4) SDS-PAGE gel

Rat PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Rat PCSK9, His TagRat PCSK9, His Tag (Cat. No. PC9-R52H4) ELISA bioactivity

Immobilized Human LDL R, Strep Tag (Cat. No. LDR-H5281) at 2 μg/mL (100 μL/well) can bind Rat PCSK9, His Tag (Cat. No. PC9-R52H4) with a linear range of 8-63 ng/mL (QC tested).

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