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Your Position: Home > Recombinant Proteins > Recombinant Human Her2 / ErbB2 / NEU Protein, Biotinylated

Biotinylated Human Her2 / ErbB2, ultra sensitivity (primary amine labeling, long spacer)

  • Synonym
    ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    MABSol® Biotinylated Human Her2 / ErbB2, Superior Sensitivity (HE2-H822R) is expressed from human HEK293 cells. It contains AA Thr 23 - Thr 652 (Accession # AAA75493). It is the biotinylated form of Human Her2 / ErbB2 Protein (Cat. No. HE2-H5225).
    Predicted N-terminus: Thr 23
  • Molecular Characterization
    Her2(Thr 23 - Thr 652) P04626-1

    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.2 kDa. The protein migrates as 95-110 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (32.5 angstroms) is used in this product to minimize potential steric hindrance.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 1.5-3.5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Her2, Superior Sensitivity (Cat. No. HE2-H822R) SDS-PAGE gel

Biotinylated Human Her2, Superior Sensitivity on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human Her2, Superior SensitivityBiotinylated Human Her2, Superior Sensitivity (Cat. No. HE2-H822R) ELISA bioactivity

Immobilized Trastuzumab at 3 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His Tag (Cat. No. HE2-H822R) with a linear range of 0.1-3.1 ng/mL (QC tested).

Human_FcRn_Heterodimer_Protein_Bli

Immobilized Herceptin on SA Biosensor via Biotinylated Her2 Protein (Cat. No. HE2-H822R), can bind Human FcRn / FCGRT & B2M Heterodimer Protein (Cat. No. FCM-H5286) with an affinity constant of 1.32 μM as determined in BLI assay (Fortebio Octet 96) ( Routinely tested).

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Price(USD) : $290.00

Inventory : In stock

Price(USD) : $1250.00

Inventory : In stock

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