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Your Position: Home > Other Recombinant Proteins > Human ENSA /Alpha-endosulfine Protein

Human Alpha-endosulfine / ENSA Protein

  • Synonym
    ENSA,Alpha-endosulfine,ARPP-19e,endosulfine Alpha
  • Source
    Human Alpha-endosulfine, His Tag (ENA-H5140) is expressed from E.coli cells. It contains AA Ser 2 - Glu 121 (Accession # AAH00436).
    Predicted N-terminus: Met
  • Molecular Characterization
    ENSA(Ser 2 - Glu 121) AAH00436

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 14.2 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Alpha-endosulfine, His Tag (Cat. No. ENA-H5140) SDS-PAGE gel

Human Alpha-endosulfine, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    Alpha-endosulfine (ENSA),a member of the endosulfine family, is also known as ARPP-19e,which is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. ENSA is a protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, ENSA is able to specifically interact with PPP2R2D (PR55-delta) and inhibit its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). ENSA acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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