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Your Position: Home > Other Recombinant Proteins > Human UBE2F Protein

Human UBE2F Protein

  • Synonym
    UBE2F,NCE2
  • Source
    Human UBE2F, His Tag (UBF-H5148) is expressed from E.coli cells. It contains AA Met 1 - Arg 185 (Accession # AAH10549).
    Predicted N-terminus: Met
  • Molecular Characterization
    UBE2F(Met 1 - Arg 185) AAH10549

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 21.9 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human UBE2F, His Tag (Cat. No. UBF-H5148) SDS-PAGE gel

Human UBE2F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    Ubiquitin-conjugating enzyme E2 F (UBE2F), a member of the ubiquitin-conjugating enzyme family and UBE2F subfamily, is also known as NEDD8-conjugating enzyme UBE2F, NEDD8 carrier protein UBE2F and NEDD8-conjugating enzyme 2 (NCE2),which can interact with UBA3 and RBX2. The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.UBE2F also can accept the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyze its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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