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Your Position: Home > All Other Proteins > UCH-L1 > Human UCHL1 Protein

Human UCH-L1 Protein

  • Synonym
    UCHL1,PGP9.5
  • Source
    Human UCH-L1, His Tag (UC1-H5140) is expressed from E.coli cells. It contains AA Met 1 - Ala 223 (Accession # NP_004172).
    Predicted N-terminus: Met
  • Molecular Characterization
    UCH-L1(Met 1 - Ala 223) NP_004172

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 25.7 kDa. The protein migrates as 26 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 500 mM Nacl, 1 mM DTT, pH8.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human UCH-L1, His Tag (Cat. No. UC1-H5140) SDS-PAGE gel

Human UCH-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ,a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain. UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also,UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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