This protein carries no "tag".
The protein has a calculated MW of 20.8 kDa. The protein migrates as 23-24 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
The sterility testing was performed by membrane filtration method described in USP <71> and Eur. Ph. 2.6.1.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
ActiveMax® Human IL-6, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Loaded Human IL-6 R alpha, Fc Tag (Cat. No. ILR-H5259) on Protein A Biosensor, can bind ActiveMax® Human IL-6, Tag Free(Cat. No. IL6-H4218) with an affinity constant of 35.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
ActiveMax® Human IL-6, Tag Free (Cat. No. IL6-H4218) stimulates proliferation of TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.2856-0.3636 ng/mL (Routinely tested).
Response to human IL-6 protein (Fold). The STAT3 (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF047) was stimulated with serial dilutions of human IL-6 protein (AcroBiosystems, Cat. No. IL6-H4218). The max induction fold was approximately 100.5 (Routinely tested).
Authors: Gao R, et al
Journal: Opt Express 2020
Application: cross activity
Authors: Z Hao, et al
Journal: Biomed Microdevices 2019
Application: aptameric graphene-based nanosensor
Authors: Z Hao, et al
Journal: Biosensors and Bioelectronics 2019
Authors: Lichtor PA, et al
Journal: Nat Chem Biol 2019
Authors: Vivek Borse, ,et al.
Journal: Sensor Actuat B-Chem 2018
Application: Fluorescence lateral flow immunoassay
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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