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Your Position: Home > Cytokines > IL-6 > ActiveMax® Human IL-6 Protein, Tag Free

ActiveMax® Human IL-6 Protein, Tag Free

  • Synonym
    IL6,Interleukin-6,BSF2,HSF,IFNB2
  • Source
    ActiveMax® Human IL-6, Tag Free (IL6-H4218) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000591).
    Predicted N-terminus: Val 30
  • Molecular Characterization
    Online(Val 30 - Met 212) NP_000591

    This protein carries no "tag".

    The protein has a calculated MW of 20.8 kDa. The protein migrates as 25-32 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose  is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
ActiveMax® Human IL-6, Tag Free (Cat. No. IL6-H4218) SDS-PAGE gel

ActiveMax® Human IL-6, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity-ELISA
ActiveMax® Human IL-6, Tag FreeActiveMax® Human IL-6, Tag Free (Cat. No. IL6-H4218) ELISA bioactivity

Immobilized ActiveMax® Human IL-6, Tag Free (Cat. No. IL6-H4218) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha, Avitag,His Tag (Cat. No. CD6-H82E8) with a linear range of 4-125 ng/mL (QC tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

ActiveMax® Human IL-6, Tag Free (Cat. No. IL6-H4218) stimulates proliferation of TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.2856-0.3636 ng/mL (Routinely tested).

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