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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > Biotinylated Human FcRN & B2M Heterodimer Protein

Biotinylated Human FcRn / FCGRT & B2M Heterodimer Protein, ultra sensitivity (primary amine labeling)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Biotinylated Human FcRn Heterodimer Protein (primary amine labeling) (FCM-H8286) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # NP_001129491.1 (FCGRT) & AAH32589 (B2M)). It is the biotinylated form of Human FcRn / FCGRT & B2M Heterodimer Protein (FCM-H5286).
    Predicted N-terminus: Ala 24(FCGRT) & Ile 21(B2M)
  • Molecular Characterization
    FcRn(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) NP_001129491.1 (FCGRT) & AAH32589 (B2M)

    Biotinylated Human FcRn Heterodimer Protein (primary amine labeling), produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 2.5-4.5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human FcRn Heterodimer Protein (primary amine labeling) (Cat. No. FCM-H8286) SDS-PAGE gel

Biotinylated Human FcRn Heterodimer Protein (primary amine labeling) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human FcRn Heterodimer Protein (primary amine labeling)Biotinylated Human FcRn Heterodimer Protein (primary amine labeling) (Cat. No. FCM-H8286) ELISA bioactivity

Immobilized Ipilimumab at 10 μg/mL (100 μL/well) can bind Biotinylated Human FcRn Heterodimer Protein (primary amine labeling) (Cat. No. FCM-H8286) with a linear range of 5-78 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Immobilized Biotinylated Human FcRn Heterodimer Protein (primary amine labeling) (Cat. No. FCM-H8286) on SA Biosensor, can bind Herceptin with an affinity constant of 0.49 μM as determined in BLI assay (Fortebio Octet 96) (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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