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Your Position: Home > MABSol® Biotin Labeled Proteins > Biotinylated Human IL6 Protein,HEK293 Expressed

Biotinylated Human IL-6, epitope tag free, ultra sensitivity (primary amine labeling)

  • Synonym
    IL6,HGF,Interleukin-6,BSF2,HSF,IFNB2
  • Source
    MABSol® Biotinylated Human IL-6 (IL6-H8218) is expressed from human HEK293 cells. It contains AA Val 30 - Met 212 (Accession # NP_000591). It is the biotinylated form of ActiveMax® Human IL-6 (Cat # IL6-H4218).
    Predicted N-terminus: Val 30
  • Molecular Characterization
    IL-6(Val 30 - Met 212) NP_000591

    The product does NOT contain any epitope tags. The protein has a calculated MW of 20.8 kDa. The protein migrates as 24-27 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 4-5.5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-6 (Cat. No. IL6-H8218) SDS-PAGE gel

Biotinylated Human IL-6 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human IL-6Biotinylated Human IL-6 (Cat. No. IL6-H8218) ELISA bioactivity

Immobilized Human IL-6 R alpha, His Tag (Cat. No. ILR-H4223) at 5 μg/mL (100 µL/well),can bind Biotinylated Human IL-6 (Cat. No. IL6-H8218) with a linear range of 4-250 ng/mL (QC tested).

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Price(USD) : $290.00

Inventory : In stock

Price(USD) : $1250.00

Inventory : In stock

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