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Your Position: Home > MABSol® Biotin Labeled Proteins > Biotinylated Human CD71 Protein

Biotinylated Human Transferrin R / CD71, ultra sensitivity (primary amine labeling)

  • Synonym
    CD71,TFRC,TFR,sTfR,TfR1,Trfr,T9,p90
  • Source
    MABSol® Biotinylated Human Transferrin R / CD71 (TFR-H8243) is expressed from human HEK293 cells. It contains AA Cys 89 - Phe 760 (Accession # AAH01188.1). It is the biotinylated form of Human Transferrin R protein, His Tag (Cat # CD1-H5243).
    Predicted N-terminus: His
  • Molecular Characterization
    TfR(Cys 89 - Phe 760) AAH01188.1

    This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 76 kDa. The protein migrates as 80-85 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 1-2 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Transferrin R (Cat. No. TFR-H8243) SDS-PAGE gel

Biotinylated Human Transferrin R on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity
Biotinylated Human Transferrin RBiotinylated Human Transferrin R (Cat. No. TFR-H8243) ELISA bioactivity

Immobilized Human Transferrin, His Tag (Cat. No. TRN-H4229) at 10 μg/mL (100 µL/well),can bind Biotinylated Human Transferrin R (Cat. No. TFR-H8243) with a linear range of 2-200 ng/mL (QC tested).

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Order Details

Price(USD) : $290.00

Inventory : In stock

Price(USD) : $1250.00

Inventory : In stock

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